The kit is based on principle of Competitive-ELISA. The kit contains micro ELISA plate coated with Human E3. available in test samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E3 are added to the micro ELISA plate wells. Human E3 in available in test samples (or standards) competes with a fixed amount of E3 on the solid phase of the plate sites on the HRP linked detection antibody specific to E3. Excess conjugate and unbound available in test sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The concentration of Human E3 available in tested samples is calculated by comparing the optical density (OD) of the samples with the standard curve.
Tested Sample Types: serum, plasma, saliva, urine Assay Time: 1.5h Cross reactivity: This kit recognizes Human E3 in samples.No significant cross-reactivity or interference between Human E3 and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8?,6 months
