Product Information

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vanillylmandelic Acid (VMA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vanillylmandelic Acid (VMA) and unlabeled Vanillylmandelic Acid (VMA) (Standards or samples) with the pre-coated antibody specific to Vanillylmandelic Acid (VMA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vanillylmandelic Acid (VMA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vanillylmandelic Acid (VMA) in the sample.

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The product is for laboratory use only (RUO).