Product Information

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenocorticotropic Hormone (ACTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated antibody specific to Adrenocorticotropic Hormone (ACTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample.

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The product is for laboratory use only (RUO).