Sensitivity: 9.38pg/mL
Detection: 15.63 - 1000pg/mL
Sample: Serum, Plasma, Cell supernatant.
Target Name: ADH
UNIProt ID:
This ELISA Kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine ADH. During the reaction, Porcine ADH in the sample or standard competes with a fixed amount of Porcine ADH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Porcine ADH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Porcine ADH in the samples is then determined by comparing the OD of the samples to the standard curve.
