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Product Information

  • Product Name Porcine AFGF/FGF1(Acidic Fibroblast Growth Factor 1) ELISA Kit
  • Catalog No. E-EL-P0583
  • Pack Size 96Wells
  • Brand Elabscience Biotechnology Inc.
  • Product Detail Porcine AFGF/FGF1(Acidic Fibroblast Growth Factor 1) ELISA Kit. Sandwich - ELISA Principle, Detection is by Colorimetric method.
  • Features

    Sensitivity: 9.38pg/mL

    Detection: 15.63~1000pg/mL

    Sample: Serum, Plasma, Cell supernatant

Sensitivity: 9.38pg/mL

Detection: 15.63 - 1000pg/mL

Sample: Serum, Plasma, Cell supernatant.

Target Name: AFGF/FGF1

UNIProt ID: P20002

This ELISA Kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine AFGF/FGF1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine AFGF/FGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine AFGF/FGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Porcine AFGF/FGF1. You can calculate the concentration of Porcine AFGF/FGF1 in the samples by comparing the OD of the samples to the standard curve.
Data Sheet:


User Manual:

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