The kit is based on principle of Sandwich-CLIA. The micro CLIA plate of the kit has been pre-coated with an antibody specific to Human 12-LO. test sample and standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a detection antibody (biotinylated) specific for Human 12-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each well of the well and then the plate is incubated for specific time. The free conjugate is washed away by buffer. The substrate solution is added to each well. The well that contains Human 12-LO, detection antibody (biotinylated) and Avidin-HRP conjugate will appear luminescence. At 420nm, the Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The obtained RLU value is positively associated with the concentration of Human 12-LO. The concentration is calculated for Human 12-LO in the samples by comparing the RLU value of the samples with the standard curve.
Tested Sample Types: Tissue homogenates,cell lysates and other biological fluids Assay Time: 3.5h Cross reactivity: This kit recognizes Human 12-LO in samples.No significant cross-reactivity or interference between Human 12-LO and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8 degree C./-20 degree C., 6 months
