The kit is based on principle of Sandwich-CLIA. The micro CLIA plate of the kit has been pre-coated with an antibody specific to Mouse PAPPA. test sample and standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a detection antibody (biotinylated) specific for Mouse PAPPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each well of the well and then the plate is incubated for specific time. The free conjugate is washed away by buffer. The substrate solution is added to each well. The well that contains Mouse PAPPA, detection antibody (biotinylated) and Avidin-HRP conjugate will appear luminescence. At 420nm, the Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The obtained RLU value is positively associated with the concentration of Mouse PAPPA. The concentration is calculated for Mouse PAPPA in the samples by comparing the RLU value of the samples with the standard curve.
Tested Sample Types: serum, plasma and other biological fluids Assay Time: 3.5h Cross reactivity: This kit recognizes Mouse PAPPA in samples.No significant cross-reactivity or interference between Mouse PAPPA and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8 degree C./-20 degree C., 6 months
