Elabscience Mouse VEGFR-2/KDR (Vascuoar Endothelial Growth Factor Receptor 2) CLIA Kit (RUO)

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Product Name Elabscience Mouse VEGFR-2/KDR (Vascuoar Endothelial Growth Factor Receptor 2) CLIA Kit (RUO)
Catalog No. E-CL-M0399
Pack Size 96T/48T
Brand Elabscience Bionovation Inc.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse VEGFR-2/KDR concentrations in serum, plasma and other biological fluids.
Features
Assay Method: Chemiluminescence

Sensitivity: 18.75 pg/mL

Detection Range: 31.25-2000 pg/mL

The kit is based on principle of Sandwich-CLIA. The micro CLIA plate of the kit has been pre-coated with an antibody specific to Mouse VEGFR-2/KDR. test sample and standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a detection antibody (biotinylated) specific for Mouse VEGFR-2/KDR and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each well of the well and then the plate is incubated for specific time. The free conjugate is washed away by buffer. The substrate solution is added to each well. The well that contains Mouse VEGFR-2/KDR, detection antibody (biotinylated) and Avidin-HRP conjugate will appear luminescence. At 420nm, the Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The obtained RLU value is positively associated with the concentration of Mouse VEGFR-2/KDR. The concentration is calculated for Mouse VEGFR-2/KDR in the samples by comparing the RLU value of the samples with the standard curve.

Tested Sample Types: serum, plasma and other biological fluids Assay Time: 3.5h Cross reactivity: This kit recognizes Mouse VEGFR-2/KDR in samples.No significant cross-reactivity or interference between Mouse VEGFR-2/KDR and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8 degree C./-20 degree C., 6 months