Product Information

Isotype: IgG1k

Formulation: PBS with 0.02% Sodium Azide.

Immunogen: A BALB/c mouse was immunized with a conjugate of bromodeoxyrudine and Helix pomatia hemocyanin (HPH).The conjugate was prepared using periodate oxidation. Fusion partner: P3-X63-Ag8.653.

Dilution range: Flow cytometry (0, 5-1, 0 ug/million cells in 0, 1 ml, fix cells in 4% PFA for 10 min, at 4?C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4?C). Immunofluorescence (0, 5-1, 0 ug/ml). Immunohistology (2-4 ug/ml for 30 min at RT; staining of formalin-fixed tissues requires pre-treatment of sections with 4N HCl for 30 minutes at RT to denature double-stranded DNA, followed by digestion with trypsin at 1 mg/ml PBS for 20 min at RT or 10 min at 37?C).

Application area: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.