Host: Mouse
Application: FC/WB/IF/IHC
Reactivity: Human/Mouse
Isotype: IgG1k
Formulation: PBS with 0.02% Sodium Azide.
Immunogen: A BALB/c mouse was immunized with a high molecular fraction of proteins secreted by cultured human fibroblasts.Fusion partner: P3-C63-Ag8.653.
Dilution range: Flow cytometry (1-2 ug/million cells in 0, 1 ml). Immunoblotting (1 ug/ml for 2h at RT). Immunofluorescence (1-2 ug/ml). Immunohistology (1-2 ug/ml for 30-60 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
Application area: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Purification: Affinity purified from tissue culture.
Storage Instruction: Store for up to 1 year at 2-8 Degree C. upon receipt.
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