Product Information

Isotype: IgG1k

Formulation: PBS with 0.02% Sodium Azide.

Immunogen: A hybrid (BALB/c x B6) mouse was immunized with a cytoskeleton preparation of human A431 carcinoma cell line. Fusion partner: Sp2/0.

Dilution range: Flow Cytometry (1-2 ug/million cells in 0, 1 ml, fix cells in 4% PFA for 10 min, at 4?C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4?C). Immunoblotting (1-2 ug/ml). Immunofluorescence (0.5-1, 0 ug/ml). Immunohistology (1-2 ug/ml for 30 min at RT; staining of formalin-fixed tissues requires 0, 1% pronase digestion (0, 1% CaCl2, 0, 1N NaOH) for 15min. at 37?C).

Application area: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.