Product Information

Isotype: IgG1k

Formulation: PBS with 0.02% Sodium Azide.

Immunogen: A BALB/c mouse was immunized with a cytoskeleton preparation of human A431 carcinoma cell line. Fusion partner: P3-X63-Ag8.653.

Dilution range: Flow Cytometry (0.5-1.0 ug/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4?C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4?C). Immunoblotting (1 ug/ml for 2h at RT). Immunofluorescence (0.5-1 ug/ml). Immunohistology (formalin-fixed: 1-2 ug/ml for 30 min at RT; requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes or digestion of sections with trypsin at 1mg/ml PBS, 15 min at RT).

Application area: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.