Product Information

Isotype: IgG1k

Formulation: PBS with 0.02% Sodium Azide.

Immunogen: A hybrid mouse (BALB/c x B6) was immunized with purified human keratin 7, isolated from HeLa cytoskeleton. Fusion partner: P3-X63-Ag8.653.

Dilution range: Flow Cytometry (1-2 ug/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4?C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4?C). Immunoblotting (1-2 ug/ml). Immunofluorescence (1-2 ug/ml). Immunohistology (formalin-fixed: 1-2 ug/ml for 30 min at RT; staining of formalin-fixed tissues requires 0, 1% pepsin digestion for 30 min. at 37?C C).

Application area: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.