Product Information

Isotype: IgG1k

Formulation: PBS with 0.02% Sodium Azide.

Immunogen: A BALB/c mouse was immunized with a cytoskeleton preparation from HeLa cells. Fusion Partner: Sp2/0.

Dilution range: Flow Cytometry (1-2 ug/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4?C C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4?C). Immunoblotting (1-2 ug/ml). Immunofluorescence (1-2 ug/ml). Immunohistology (2-4 ug/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, or digestion with trypsin at 1 mg/ml PBS, 15 min at RT).

Application area: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.