Host: Rat
Application: FC/WB/IF/IHC/IP
Reactivity: Mouse
Isotype: IgGk
Formulation: PBS with 0.02% Sodium Azide.
Immunogen: A Wistar rat was immunized with mouse lymph node cells. Fusion partner: Sp2/0-Ag14.
Dilution range: Flow cytometry (0, 5-1, 0 ug/million cells in 0, 1 ml). Immunoblot (1-2 ug/ml). Immunofluorescence (0, 5-1, 0 ug/ml). Immunohistology (1-2 ug/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Immunoprecipitation (1-2 ug per 100-500 ug cell lysate protein/1 ml of anti-mouse coated Sepharose-4B suspension).
Application area: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Purification: Affinity purified from tissue culture.
Storage Instruction: Store for up to 1 year at 2-8 Degree C. upon receipt.
Feel free to ask Query