Host: Mouse
Application: ELISA/FC/WB/IF/IHC
Reactivity: Human/Rat
Isotype: IgG1 Kappa
Formulation: PBS with 0.02% Sodium Azide.
Immunogen: A BALB/c mouse was immunized with human rTNF-alpha. Fusion partner: NS-1.
Dilution range: ELISA (solid phase: 0, 1-100 ug/ml; tracer: 0, 001-100 ug/ml for 30 min at RT). Flow cytometry (0, 5-1, 0 ug/million cells in 0.1ml). Immunoblotting (1-2 ug/ml). Immunofluorescence (1-2 Mu g/ml). Inhibits tumor growth (order Ab without azide). Immunohistology (1-2 ug/ml for 30-60 minutes at RT; for staining of formalin-fixed tissues no suitable antigen retrieval method is known to date). Neutralizes rTNF-alpha mediated cytotoxicity (order Ab without azide). Protects mice against toxicity of rhTNF-alpha (order Ab without azide).
Application area: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Purification: Affinity purified from tissue culture.
Storage Instruction: Store for up to 1 year at 2-8 Degree C. upon receipt.
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