The kit is based on principle of Competitive-ELISA. The kit contains micro ELISA plate coated with Canine Cortisol. available in test samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Canine Cortisol are added to the micro ELISA plate wells. Canine Cortisol in available in test samples (or standards) competes with a fixed amount of Cortisol on the solid phase of the plate sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound available in test sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The concentration of Canine Cortisol available in tested samples is calculated by comparing the optical density (OD) of the samples with the standard curve.
Tested Sample Types: serum, plasma Assay Time: 1.5h Cross reactivity: This kit recognizes Canine Cortisol in samples.No significant cross-reactivity or interference between Canine Cortisol and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8?,6 months
