Elabscience HS Mouse IFN-Y (Interferon Gamma) ELISA Kit (RUO)

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Product Name Elabscience HS Mouse IFN-Y (Interferon Gamma) ELISA Kit (RUO)
Catalog No. E-HSEL-M0007
Pack Size 96T/48T
Brand Elabscience Bionovation Inc.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-? concentrations in serum, plasma and other biological fluids.
Features
Assay Method: Colormetric

Sensitivity: 0.84 pg/mL

Detection Range: 1.56-100 pg/mL

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate of the kit has been pre-coated with an antibody specific to Mouse IFN-?. Test samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a detection antibody (biotinylated) specific for Mouse IFN-? and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each well of the well and then the plate is incubated for specific time. The free conjugate is washed away by buffer. The substrate solution is added to each well. The well that contains Mouse IFN-?, detection antibody (biotinylated) and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. In the last, the optical density (OD) of the wells are measured by spectrophotometer at a wavelength of 450 nm ? 2 nm. The OD value comes in proportional to the concentration of Mouse IFN-?. The concentration is calculated for Mouse IFN-? in the samples by comparing the OD of the samples with the standard curve.

Tested Sample Types: serum, plasma and other biological fluids Assay Time: 3.5h Cross reactivity: This kit recognizes Mouse IFN-? in samples.No significant cross-reactivity or interference between Mouse IFN-? and analogues was observed Precision: Storage and Expiry: 2-8 degree C./-20 degree C., 12 months