This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate of the kit has been pre-coated with an antibody specific to Human ?FP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human ?FP are added to the micro ELISA plate wells. Human ?FP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human ?FP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The OD value comes in proportional to the concentration of Human ?FP. The concentration of Human ?FP in the samples is then determined by comparing the OD of the samples with the standard curve.
Tested Sample Types: serum, plasma Assay Time: 1.5h Cross reactivity: This kit recognizes Human ?FP in samples.No significant cross-reactivity or interference between Human ?FP and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8?,6 months
