This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate of the kit has been pre-coated with an antibody specific to Human ADP/Acrp30. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a detection antibody (biotinylated) specific for Human ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each well of the well and then the plate is incubated for specific time. The free conjugate is washed away by buffer. The substrate solution is added to each well. The well that contains Human ADP/Acrp30, detection antibody (biotinylated) and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ? 2 nm. The OD value comes in proportional to the concentration of Human ADP/Acrp30. The concentration is calculated for Human ADP/Acrp30 in the samples by comparing the OD of the samples with the standard curve.
Tested Sample Types: serum, plasma Assay Time: 2.5h Cross reactivity: This kit recognizes Human ADP/Acrp30 in samples.No significant cross-reactivity or interference between Human ADP/Acrp30 and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8?,6 months
