This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate of the kit has been pre-coated with an antibody specific to Mouse CLU. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse CLU are added to the micro ELISA plate wells. Mouse CLU in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse CLU.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The OD value comes in proportional to the concentration of Mouse CLU. The concentration of Mouse CLU in the samples is then determined by comparing the OD of the samples with the standard curve.
Tested Sample Types: serum, plasma and other biological fluids Assay Time: 1.5h Cross reactivity: This kit recognizes Mouse CCL6 in samples. No significant cross-reactivity or interference between Mouse CCL6 and analogues was observed. Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8?,6 months
