This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate of the kit has been pre-coated with an antibody specific to Mouse IgG3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG3 are added to the micro ELISA plate wells. Mouse IgG3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG3.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The OD value comes in proportional to the concentration of Mouse IgG3. The concentration of Mouse IgG3 in the samples is then determined by comparing the OD of the samples with the standard curve.
Tested Sample Types: serum, plasma Assay Time: 1.5h Cross reactivity: This kit recognizes Mouse IgG3 in samples.No significant cross-reactivity or interference between Mouse IgG3 and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8?,6 months
