The kit is based on principle of Competitive-ELISA. The kit contains micro ELISA plate coated with Rabbit Pg. available in test samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rabbit Pg are added to the micro ELISA plate wells. Rabbit Pg in available in test samples (or standards) competes with a fixed amount of Pg on the solid phase of the plate sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound available in test sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The concentration of Rabbit Pg available in tested samples is calculated by comparing the optical density (OD) of the samples with the standard curve.
Tested Sample Types: serum, plasma Assay Time: 1.5h Cross reactivity: This kit recognizes Rabbit Pg in samples.No significant cross-reactivity or interference between Rabbit Pg and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8?,6 months
