This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate of the kit has been pre-coated with an antibody specific to Rat ADP/Acrp30. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ADP/Acrp30 are added to the micro ELISA plate wells. Rat ADP/Acrp30 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ADP/Acrp30.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The OD value comes in proportional to the concentration of Rat ADP/Acrp30. The concentration of Rat ADP/Acrp30 in the samples is then determined by comparing the OD of the samples with the standard curve.
Tested Sample Types: serum, plasma Assay Time: 1.5h Cross reactivity: This kit recognizes Rat ADP/Acrp30 in samples.No significant cross-reactivity or interference between Rat ADP/Acrp30 and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8?,6 months
