Elabscience Rat Cys-C (Cystatin C) ELISA Kit (RUO)

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Product Name Elabscience Rat Cys-C (Cystatin C) ELISA Kit (RUO)
Catalog No. E-EL-R0304
Pack Size 96T/48T
Brand Elabscience Bionovation Inc.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Cys-C concentrations in serum, plasma and other biological fluids.
Features
Assay Method: Colormetric

Sensitivity: 0.1 ng/mL

Detection Range: 0.16-10 ng/mL

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate of the kit has been pre-coated with an antibody specific to Rat Cys-C. Test samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a detection antibody (biotinylated) specific for Rat Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each well of the well and then the plate is incubated for specific time. The free conjugate is washed away by buffer. The substrate solution is added to each well. The well that contains Rat Cys-C, detection antibody (biotinylated) and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. In the last, the optical density (OD) of the wells are measured by spectrophotometer at a wavelength of 450 nm ? 2 nm. The OD value comes in proportional to the concentration of Rat Cys-C. The concentration is calculated for Rat Cys-C in the samples by comparing the OD of the samples with the standard curve.

Tested Sample Types: serum, plasma and other biological fluids Assay Time: 3.5h Cross reactivity: This kit recognizes Rat Cys-C in samples. No significant cross-reactivity or interference between Rat Cys-C and analogues was observed Precision: Both intra-CV and inter-CV are < 10%.Storage and Expiry: 2-8 degree C./-20 degree C., 12 months