This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Spike protein IgM in the sample. The micro ELISA plate of the kit is pre-coated with purified SARS-CoV-2 Spike protein (PKSR030489) antigen, after adding samples to wells, the SARS-CoV-2 Spike protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Spike protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. The free conjugate is washed away by buffer. , then the substrate solution is added to each well. The well that contains SARS-CoV-2 Spike protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ? 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike protein IgM exists in the tested samples or not.
Tested Sample Types: serum, plasma Assay Time: 1.5h Cross reactivity: This kit recognizes Human SARS-CoV-2 IgM in samples.No significant cross-reactivity or interference between Human SARS-CoV-2 IgM and analogues was observed Precision: Intra-CV: CV%?8%; Inter-CV: CV%?10%Storage and Expiry: 2-8?,12 months
