This ELISA kit uses the Sandwich-Ag ELISA principle. The micro ELISA plate of the kit has been pre-coated with recombinant SARS-CoV-2 Spike Protein. After adding samples (or controls) to wells, the total antibodies against SARS-CoV-2 Spike Protein will combine with the pre-coated SARS-CoV-2 Spike Protein. After washing completely, add Horseradish Peroxidase (HRP) conjugated SARS-CoV-2 Spike Protein to develop the antigen-antibody-HRP conjugated antigen complex. The free conjugate is washed away by buffer. The substrate solution is added to each well. The well that contains SARS-CoV-2 Spike Protein, antibodies against SARS-CoV-2 Spike Protein and HRP conjugated SARS-CoV-2 Spike Protein will appear blue in color. The enzyme-substrate reaction is completed by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ? 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike Protein total antibody exists in the tested samples or not.
Tested Sample Types: serum, plasma Assay Time: 3.5h Cross reactivity: This kit recognizes SARS-CoV-2 Spike Protein Total Antibody in samples.No significant cross-reactivity or interference between Human SARS-CoV-2 Neutralization Antibody and analogues was observed Precision: Intra-CV: CV%?8%; Inter-CV: CV%?10%Storage and Expiry: 2-8 degree C./-20 degree C., 12 months
