LycoMark 1-Deoxy-D-glucose 1-Deoxy-D-glucopyranose 1,5-Anhydro-D-glucitol 1,5-Anhydrosorbitol Aceritol PolygalytolELK Biotechnology 1,5-AG(1,5-Anhydroglucitol) ELISA Kit

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Product Name ELK Biotechnology 1,5-AG(1,5-Anhydroglucitol) ELISA Kit
Catalog No. ELK7840
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General 1,5-Anhydroglucitol concentrations in serum, plasma and other biological fluids
Features
Sensitivity: 0.6 ?g/mL

Standard: 150 ?g/mL

Detection Range: 2.35-150 ?g/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism;

Alternate Names: LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve.