4-HNE HNE 4-hydroxy-2-nonenal 4-HydroxynonenalELK Biotechnology 4-HNE(4-Hydroxynonenal) ELISA Kit

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Product Name ELK Biotechnology 4-HNE(4-Hydroxynonenal) ELISA Kit
Catalog No. ELK9581
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General 4-Hydroxynonenal concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.38 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

Alternate Names: 4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.