Oxitriptan Cincofarm Levothym Levotonine Oxyfan Telesol Tript-OH TriptumELK Biotechnology 5-HTP(5-Hydroxytryptophan) ELISA Kit

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Product Name ELK Biotechnology 5-HTP(5-Hydroxytryptophan) ELISA Kit
Catalog No. ELK8124
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General 5-Hydroxytryptophan concentrations in serum, plasma and other biological fluids
Features
Sensitivity: 1.77 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Enzyme & Kinase;

Alternate Names: Oxitriptan; Cincofarm; Levothym; Levotonine; Oxyfan; Telesol; Tript-OH; Triptum

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HTP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HTP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of 5-HTP in the samples is then determined by comparing the OD of the samples to the standard curve.