5-Me-THFA 5-Me-H4FA 5-Me-THF 5-Me-H4F Levomefolic Acid Metafolin 5-Methyltetrahydrofolic Acid L-MethylfolateELK Biotechnology 5-Me-THF(5-Methyltetrahydrofolate) ELISA Kit

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Product Name ELK Biotechnology 5-Me-THF(5-Methyltetrahydrofolate) ELISA Kit
Catalog No. ELK8059
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General 5-Methyltetrahydrofolate concentrations in serum, plasma and other biological fluids
Features
Sensitivity: 0.43 ng/mL

Standard: 90 ng/mL

Detection Range: 1.41-90 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Hematology;Reproductive science;Nutrition metabolism;

Alternate Names: 5-Me-THFA; 5-Me-H4FA; 5-Me-THF; 5-Me-H4F; Levomefolic Acid; Metafolin; 5-Methyltetrahydrofolic Acid; L-Methylfolate

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Me-THF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Me-THF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of 5-Me-THF in the samples is then determined by comparing the OD of the samples to the standard curve.