N,N-Dimethylarginine NG,NG-Dimethylarginine dihydrochloride Asymmetric DimethylarginineELK Biotechnology ADMA(Asymmetrical Dimethylarginine) ELISA Kit

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Product Name ELK Biotechnology ADMA(Asymmetrical Dimethylarginine) ELISA Kit
Catalog No. ELK7845
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General Asymmetrical Dimethylarginine concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 4.51 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;Cardiovascular biology;

Alternate Names: N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ADMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ADMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of ADMA in the samples is then determined by comparing the OD of the samples to the standard curve.