c-AMP 3'-5'-Cyclic Adenosine Monophosphate Adenosine CyclophosphateELK Biotechnology cAMP(Cyclic Adenosine Monophosphate) ELISA Kit

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Product Name ELK Biotechnology cAMP(Cyclic Adenosine Monophosphate) ELISA Kit
Catalog No. ELK8116
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General Cyclic Adenosine Monophosphate concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 92.5 pg/mL

Standard: 20000 pg/mL

Detection Range: 312.5-20000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Signal transduction;Metabolic pathway;

Alternate Names: c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cAMP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.