BPTI Trasylol Pancreatic Trypsin Inhibitor Basic protease inhibitorELK Biotechnology Cattle AP(Aprotinin) ELISA Kit

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Product Name ELK Biotechnology Cattle AP(Aprotinin) ELISA Kit
Catalog No. ELK6113
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Cattle Aprotinin concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.55 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Enzyme & Kinase;Cardiovascular biology;Human gene deletion;Hepatology;

Alternate Names: BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle AP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Cattle AP in the samples is then determined by comparing the OD of the samples to the standard curve.