Hgb Haemoglobin Heterotetramer(??)2ELK Biotechnology Cattle HB(Hemoglobin) ELISA Kit

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Product Name ELK Biotechnology Cattle HB(Hemoglobin) ELISA Kit
Catalog No. ELK8165
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Cattle Hemoglobin concentrations in Serum, plasma, erythrocyte lysates and other biological fluids
Features
Sensitivity: 5.57 ?g/mL

Standard: 1000 ?g/mL

Detection Range: 15.63-1000 ?g/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science;

Alternate Names: Hgb; Haemoglobin; Heterotetramer(??)2

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle HB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Cattle HB in the samples is then determined by comparing the OD of the samples to the standard curve.