Pregnancy-associated glycoprotein 1 PAG 1 EC 3.4.23. Pregnancy-specific protein B PSP-BELK Biotechnology Cattle PSPB(Pregnancy Specific Protein B) ELISA Kit

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Product Name ELK Biotechnology Cattle PSPB(Pregnancy Specific Protein B) ELISA Kit
Catalog No. ELK0478
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Cattle Pregnancy Specific Protein B concentrations in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.28 ng/mL

Standard: 50 ng/mL

Detection Range: 0.78-50 ng/mL

Assay Length: 2.5h

Assay Type: Competitive Inhibition

Research Area: Signal transduction; Cardiovascular; Cancer; Metabolism

Alternate Names: Pregnancy-associated glycoprotein 1; PAG 1; EC 3.4.23.; Pregnancy-specific protein B; PSP-B

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle PSPB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PSPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Cattle PSPB in the samples is then determined by comparing the OD of the samples to the standard curve.