c-GMP 3',5'-Cyclic GMP Cyclic 3',5'-GMP Guanosine 3',5'-Cyclic PhosphateELK Biotechnology cGMP(Cyclic Guanosine Monophosphate) ELISA Kit

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Product Name ELK Biotechnology cGMP(Cyclic Guanosine Monophosphate) ELISA Kit
Catalog No. ELK8144
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General Cyclic Guanosine Monophosphate concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 45.2 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Signal transduction;Metabolic pathway;

Alternate Names: c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cGMP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cGMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.