GCG GLP1 GLP2 GRPP Glicentin-Related Polypeptide Glucagen Oxyntomodulin Incretin hormoneELK Biotechnology Chicken GC(Glucagon) ELISA Kit

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Product Name ELK Biotechnology Chicken GC(Glucagon) ELISA Kit
Catalog No. ELK5744
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Chicken Glucagon concentrations in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Features
Sensitivity: 7.59 pg/mL

Standard: 1500 pg/mL

Detection Range: 23.44-1500 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Endocrinology;

Alternate Names: GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken GC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Chicken GC in the samples is then determined by comparing the OD of the samples to the standard curve.