L-Reduced GlutathioneELK Biotechnology Chicken GSH(Glutathione) ELISA Kit

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Product Name ELK Biotechnology Chicken GSH(Glutathione) ELISA Kit
Catalog No. ELK0593
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Chicken Glutathione concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluidstissue homogenates
Features
Sensitivity: 0.48 ng/mL

Standard: 80 ng/mL

Detection Range: 1.25-80 ng/mL

Assay Length: 2.5h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;

Alternate Names: L-Reduced Glutathione

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken GSH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Chicken GSH in the samples is then determined by comparing the OD of the samples to the standard curve.