DH-VD3 Calcitriol Rocaltrol Calcijex 1,25-(OH)2D3 1,25-Dihydroxycholecalciferol 1,25-Dihydroxy Vitamin D3ELK Biotechnology DHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit

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Product Name ELK Biotechnology DHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit
Catalog No. ELK8091
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General 1,25-Dihydroxyvitamin D3 concentrations in serum, plasma and other biological fluids
Features
Sensitivity: 49.5 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;Bone metabolism;

Alternate Names: DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHVD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.