AM PAMP Proadrenomedullin N-20 terminal peptide ProAM N-terminal 20 peptideELK Biotechnology Dog ADM(Adrenomedullin) ELISA Kit

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Product Name ELK Biotechnology Dog ADM(Adrenomedullin) ELISA Kit
Catalog No. ELK5647
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Dog Adrenomedullin concentrations in Serum, plasma and other biological fluids.
Features
Sensitivity: 4.42 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Endocrinology;Hormone metabolism;

Alternate Names: AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog ADM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Dog ADM in the samples is then determined by comparing the OD of the samples to the standard curve.