iPTH Intact Parathyroid Hormone Parathormone Parathyrin PTH Parathyroid HormoneELK Biotechnology Dog I-PTH(IntactParathormone) ELISA Kit

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Product Name ELK Biotechnology Dog I-PTH(IntactParathormone) ELISA Kit
Catalog No. ELK6044
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Dog IntactParathormone concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 4.15 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;

Alternate Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog I-PTH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Dog I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.