NT-Pro-BNP N-BNP ANFB_HUMAN BNP(1-32) BNP(5-29) BNP-32 Gamma-brain natriuretic peptide nppbELK Biotechnology Dog NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

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Product Name ELK Biotechnology Dog NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Catalog No. ELK7307
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Dog N-Terminal Pro-Brain Natriuretic Peptide concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 24.2 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Endocrinology;Cardiovascular biology;

Alternate Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog NT-ProBNP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Dog NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.