Collagen Type 3 Col 3 Collagen Type III Col IIIELK Biotechnology Horse COL3(Collagen Type III) ELISA Kit

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Product Name ELK Biotechnology Horse COL3(Collagen Type III) ELISA Kit
Catalog No. ELK8221
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Horse Collagen Type III concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 4.98 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;Cardiovascular biology;

Alternate Names: Collagen Type 3; Col 3; Collagen Type III; Col III

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Horse COL3 in the samples is then determined by comparing the OD of the samples to the standard curve.