SLC52A2 PAR1 GPCR41 RFT3 Porcine en?enous retrovirus A receptor 1 Riboflavin transporter 3 Solute carrier family 52, riboflavin transporter, member 2ELK Biotechnology Human GPR172A(G Protein Coupled Receptor 172A) ELISA Kit

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Product Name ELK Biotechnology Human GPR172A(G Protein Coupled Receptor 172A) ELISA Kit
Catalog No. ELK5023
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Human G Protein Coupled Receptor 172A concentrations in Tissue homogenates, saliva and other biological fluids.
Features
Sensitivity: 0.57 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: CD & Adhesion molecule;

Alternate Names: SLC52A2; PAR1; GPCR41; RFT3; Porcine en?enous retrovirus A receptor 1; Riboflavin transporter 3; Solute carrier family 52, riboflavin transporter, member 2

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPR172A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPR172A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPR172A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human GPR172A in the samples is then determined by comparing the OD of the samples to the standard curve.