Thing2 dHand Hed bHLHa26 Class A basic helix-loop-helix protein 26 Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2ELK Biotechnology Human HAND2(Heart And Neural Crest Derivatives Expressed Protein 2) ELISA Kit

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Product Name ELK Biotechnology Human HAND2(Heart And Neural Crest Derivatives Expressed Protein 2) ELISA Kit
Catalog No. ELK5167
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Human Heart And Neural Crest Derivatives Expressed Protein 2 concentrations in Tissue homogenates, cell lysates and other biological fluids.
Features
Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Cardiovascular biology;

Alternate Names: Thing2; dHand; Hed; bHLHa26; Class A basic helix-loop-helix protein 26; Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HAND2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HAND2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HAND2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human HAND2 in the samples is then determined by comparing the OD of the samples to the standard curve.