Assay Type: Sandwich
Research Area: Signal transduction;
Alternate Names: NKX6A; Nkx6.1; NK6 Transcription Factor Related,Locus 1; Homeobox protein NK-6 homolog A
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX6-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX6-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX6-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human NKX6-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
