P3NP N-Propeptide Of Type III Procollagen Procollagen III Amino Terminal PropeptideELK Biotechnology Human PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit

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Product Name ELK Biotechnology Human PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit
Catalog No. ELK5418
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Human Procollagen III N-Terminal Propeptide concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 2.13 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;Hepatology;Bone metabolism;

Alternate Names: P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIIINP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIIINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human PIIINP in the samples is then determined by comparing the OD of the samples to the standard curve.