PP2C-beta-X PP2CB PP2CBETA PPC2BETAX Protein Phosphatase 2C,Beta Isoform Protein Phosphatase 1B(formerly 2C),Magnesium-Dependent,Beta IsoformELK Biotechnology Human PPM1B(Protein Phosphatase, Mg2+/Mn2+ Dependent 1B) ELISA Kit

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Product Name ELK Biotechnology Human PPM1B(Protein Phosphatase, Mg2+/Mn2+ Dependent 1B) ELISA Kit
Catalog No. ELK5400
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Human Protein Phosphatase, Mg2+/Mn2+ Dependent 1B concentrations in tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;

Alternate Names: PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX; Protein Phosphatase 2C,Beta Isoform; Protein Phosphatase 1B(formerly 2C),Magnesium-Dependent,Beta Isoform

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPM1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPM1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPM1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human PPM1B in the samples is then determined by comparing the OD of the samples to the standard curve.