HOC6H3(OCH3)CH2COOH 3-Methoxy-4-hydroxyphenyl Acetic Acid 4-Hydroxy-3-Methoxy-Benzeneacetic Acid 4-Hydroxy-3-Methoxyphenylacetic AcidELK Biotechnology HVA(Homovanillic Acid) ELISA Kit

Add to Enquire list

Product Name ELK Biotechnology HVA(Homovanillic Acid) ELISA Kit
Catalog No. ELK7921
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General Homovanillic Acid concentrations in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.97 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Neuroscience

Alternate Names: HOC6H3(OCH3)CH2COOH; 3-Methoxy-4-hydroxyphenyl Acetic Acid; 4-Hydroxy-3-Methoxy-Benzeneacetic Acid; 4-Hydroxy-3-Methoxyphenylacetic Acid

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of HVA in the samples is then determined by comparing the OD of the samples to the standard curve.